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10 Sep 2019

The Spore-Quantum thesis starts

The SPORES-QUANTUM thesis project, supported by the Carnot Qualiment Institute's call for projects and by ANRT via the Cifre system, involves three partners: the CTCPA of Avignon Unité EMaiRIT'S (Dr. Nathalie Paniel, project leader), the BAE team of the University of Via Domitia in Perpignan (Prs. Lise Barthelmebs & Thierry Noguer) as well as the STLO laboratory of INRA in Rennes (Dr. Eric Guédon).

Spores-Quantum aims to limit financial losses and food waste in the canning industries (dairy products and green vegetables) by preventing microbiological non-stabilities induced under certain storage conditions by excessive spore contamination of thermophilic spore alteration bacteria, Geobacillus stearothermophilus and Moorella thermoacetica.

To do this, two apta sensors dedicated to the detection or even quantification of G. stearothermophilus and M. thermoacetica spores, respectively, will be developed. The spore recognition element will be an aptamer (specific to each strain), i.e. a synthetic DNA obtained by the Systematic Evolution of Ligands by EXponential enrichment technology: SELEX.

For each species, an electrochemically detected biosensor and an optical detection biosensor of the "strip test" type (strip-test or LFA) will be developed. These apta sensors will allow real-time food matrix analyses to be carried out in order to quickly qualify batches of raw materials in terms of contamination before heat treatment and line diagnostics.

 

Contact : Dr Nathalie Paniel

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